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AN AUTOMATED, RAPID, AND QUANTITATIVE MICROASSAY FOR HISTOCOMPATIBILITY TESTING

1973; Wolters Kluwer; Volume: 16; Issue: 5 Linguagem: Inglês

10.1097/00007890-197311000-00004

1534-6080

Merlin R. Wilson, J. F. Dardano, Henry Rothschild,

Animal testing and alternatives

The lymphocytotoxicity microassay of histocompatibility testing was quantitated by the use of a new instrument, the Cytograf 6301. Reconstitution experiments and comparison with microscopic observations by technicians confirmed the sensitivity, accuracy, and reproducibility of the instrument. Measurements of HL-A antigenicity assayed on a microscale by the instrument were qualitatively similar to the standard microscopic microdroplet method. Moreover, the instrument allowed rapid and automated quantitation of cytotoxic activity.