Concanavalin A Improves the Polyethylene Glycol Method for Fusing Plant Protoplasts
1978; Wiley; Volume: 44; Issue: 2 Linguagem: Inglês
10.1111/j.1399-3054.1978.tb01620.x
ISSN1399-3054
AutoresKristina Glimelius, Anita Wallin, Torsten Eriksson,
Tópico(s)Plant Reproductive Biology
ResumoAbstract An improved method for polyethylene glycol‐induced fusion of protoplasts isolated from auxotrophic mutants of Nicotiana tabacum L. cv. Gatersleben was developed. By using Petri dishes coated with concanavalin A the attachment of protoplasts induced by polyethylene glycol was strengthened. As a consequence of the stronger attachment more fusion products remained after the dilution and washing procedure. It was also found that a shorter treatment combined with a more rapid dilution of the polyethylene glycol solution was superior to previous fusion methods used for plant protoplasts.
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