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Rab11 Is Associated with Transferrin-Containing Recycling Compartments in K562 Cells

1997; Elsevier BV; Volume: 239; Issue: 2 Linguagem: Inglês

10.1006/bbrc.1997.7520

1090-2104

Elizabeth G. Green, Elizabeth Ramm, Nikki M. Riley, David Spiro, James R. Goldenring, Marianne Wessling‐Resnick,

RNA regulation and disease

3′RACE PCR was used to survey Rab transcripts synthesized by the human hematopoietic K562 cell line. Among the identified GTP-binding proteins, Rab11 was discovered. This result was unexpected since Rab11 previously had been found associated with polarized and secretory cells. Rab11 mRNA was abundant compared to that for other Rabs in K562 cells; protein levels represented 0.05-0.1% of total membrane protein. Localization of Rab11 using confocal immunofluoresence microscopy revealed extensive overlap with transferrin in recycling and/or exocytic compartments and suggests that Rab11 in non-polarized and non-secretory cells may play a role in the trafficking and recycling of internalized proteins.