Portal de Periódicos da CAPES

Developmental toxicity of formate and formic acid in whole embryo culture: A comparative study with mouse and rat embryos

1995; Wiley; Volume: 51; Issue: 4 Linguagem: Inglês

10.1002/tera.1420510409

2472-1727

James E. Andrews, M. Ebron‐McCoy, Robert J. Kavlock, John M. Rogers,

Aldose Reductase and Taurine

Abstract Acute methanol (MeOH) toxicity in primates is attribute to the conversion of MeOH to formate and the resulting acidosis. MeOH has been shown to be developmentally toxic in mice and rats both in vivo and in vivo, but rodents neither accumulate formate nor develop acidosis after MeOH exposure. To further assess the potential human developmental effects of sodium (Na) formate and formic acid in rodent whole embryos were cultured (WEC). Day 9 rat embryos were cultured for 24 or 48 hours and day 8 mouse embyos were cultured for 24 hours in the presence of Na‐formate or formic acid. Rat and mouse embryos exposed to either agent for 24 hours exhibited a trend toward reduced growth and development and the number of abnormalities increased at the higher concentrations. Rat embryos exposed for 48 hours to either Na‐formate or formic acid showed a trend toward reduced growth and development with increasing concentrations. The anomalies abserved in both species after exposure to either compound were primarily open anterior and posterior neuropore with less frequent incidence of rotational defects, tail anomalies, enlarged pericardium and delayed heart development. Exposure to Na‐formate or formic acid for comparable periods of time results in comparable degrees of embryotoxicity at concentrations (mMolar) at least 4‐fold lower than those previously reported for methanol exposure. © 1995 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.