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Cell Kinetic in Epithelium and Stroma of Benign Prostatic Hyperplasia

1997; Lippincott Williams & Wilkins; Volume: 158; Issue: 1 Linguagem: Inglês

10.1097/00005392-199707000-00074

1527-3792

Siegfried Claus, R. Berges, Theodor Senge, Harald Schulze,

Urinary Bladder and Prostate Research

No AccessJournal of UrologyInvestigative Urology1 Jul 1997Cell Kinetic in Epithelium and Stroma of Benign Prostatic Hyperplasia Siegfried Claus, Richard Berges, Theodor Senge, and Harald Schulze Siegfried ClausSiegfried Claus , Richard BergesRichard Berges , Theodor SengeTheodor Senge , and Harald SchulzeHarald Schulze View All Author Informationhttps://doi.org/10.1097/00005392-199707000-00074AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail The induction of benign prostatic hyperplasia (BPH) from normal prostate is obviously associated with a distinct increase in epithelial and stromal proliferation. We have shown previously that the further increase of BPH volume in aging men is not associated with a further increase in proliferation. We studied whether an imbalance between programmed cell death (apoptosis) and cell proliferation may explain continuing growth in aging men. In prostates of 17 men with BPH removed by open prostatectomy proliferating cells were localized immunohistochemically with the Ki-67 antibody. Apoptotic cells were detected with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) method. Proliferation and apoptotic index was calculated with a computer assisted image analysis system. Mean proliferation index +/− standard deviation in epithelium (0.142 +/− 0.097) and stroma (0.121 +/− 0.082) was nearly identical. Mean apoptotic index in epithelium (0.172 +/− 0.156) was negligibly higher than the corresponding proliferation index. In stroma, however, no apoptotic cells were detectable. Proliferation index and apoptotic index in epithelium and proliferation index in stroma showed no correlation to patient age or prostate volume. In the epithelium of BPH, obviously the cell kinetic is balanced. On the other hand, our results indicate stromal growth due to cell proliferation in the absence of cell death. This may explain the continuous increase of BPH volume in aging men. References 1 : Growth factors affecting normal and malignant prostatic cells. In: Growth Factors in Reproduction. Edited by . New York: Springer Verlag1991: 167. Google Scholar 2 : Antagonistic effect of androgen on prostatic cell death.. Prostate1984; 5: 545. Google Scholar 3 : Implication of cell kinetic changes during the progression of human prostate cancer.. Clin. Can. Res.1995; 1: 473. Google Scholar 4 : The effect of castration on benign hypertrophy of the prostate in man.. J. Urol.1940; 43: 705. Abstract, Google Scholar 5 : Benign hypertrophy and carcinoma of the prostate.. Surgery1944; 16: 152. Google Scholar 6 : The etiology of benign prostatic hypertrophy.. Bull. N. Y. Acad. Med.1947; 25: 696. Google Scholar 7 : The development of human benign prostatic hyperplasia with age.. J. Urol.1984; 132: 474. Link, Google Scholar 8 : Benign prostatic hyperplasia. In: Campbell's Urology. Edited by . Fifth Edition. Philadelphia: W. B. Saunders1986: 1248. Google Scholar 9 : Immunohistochemical determination of age related proliferation rates in normal and benign hyperplastic human prostates.. Urol. Res.1993; 21: 305. Google Scholar 10 : Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.. Br. J. Cancer1972; 26: 239. Google Scholar 11 : Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.. J. Cell Biol.1992; 119: 493. Google Scholar 12 : Pathologie der ableitenden Harnwege und der Prostata. Berlin, Heidelberg, New York, London, Paris, Tokyo: Springer-Verlag1989: 196. Google Scholar 13 : Receptor localization with the microscope. In: Neurotransmitter Receptor Binding. 2nd Edition. Edited by . New York: Raven Press1985: 153. Google Scholar 14 : A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies.. Am. J. Clin. Pathol.1981; 75: 734. Google Scholar 15 : Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.. J. Histochem. Cytochem.1981; 29: 577. Google Scholar 16 : The proof and measurement of association between two things.. Amer. J. Psychol.1904; 15: 72. Google Scholar 17 : Nichtparametrische statistische Methoden. Berlin, New York: Walter de Gruyter1978: 250. Google Scholar 18 : Etiology and disease process of benign prostatic hyperplasia.. Prostate1989; 2: 33. Google Scholar 19 : An overview of current concepts in the study of benign prostatic hyperplasia. In: Benign Prostatic Hyperplasia. NIH publication 87-2881. Edited by . Bethesda, Md.: National Institutes of Health1985: 1. Google Scholar 20 : Evaluation of average life span of epithelial and stromal cells of human prostate by superoxide dismutase activity.. Prostate1989; 15: 263. Google Scholar 21 : The aging process.. Proc. Natl. Acad. Sci. USA1981; 78: 7124. Google Scholar 22 : Is cell aging caused by respiration-dependent injury to the mitochondrial genome?. Gerontol.1982; 28: 44. Google Scholar 23 : Lipid peroxidation and metabolism in aging: a biological, chemical, and medical approach.. Rev. Biol. Res. Aging1983; 1: 315. Google Scholar 24 : The DNA damage hypothesis of aging: importance of oxidative damage. In: . New York: Alan R. Liss, Inc.1987: 451. Google Scholar 25 : Superoxide dismutase. An enzyme function for erythrocuprein (hemocuprein).. J. Biol. Chem.1969; 244: 6049. Google Scholar 26 : Superoxide radical: an endogenous toxicant.. Ann. Rev. Pharmacol. Toxicol.1983; 23: 239. Google Scholar 27 : Human benign prostatic hyperplasia: a stromal disease? New perspectives by quantitative morphology.. Urology1980; 14: 625. Google Scholar 28 : Light microscopic stereological analysis of the normal human prostate and of benign prostatic hyperplasia.. J. Urol.1979; 122: 487. Abstract, Google Scholar 29 : Role of programmed cell death in carcinogenesis. Environ. Health Perspect.1993; 101: 27. Google Scholar 30 : Adrenocortical cell deletion: the role of ACTH.. J. Pathol.1973; 111: 85. Google Scholar 31 : Relationship between DNA fragmentation and apoptosis in the programmed cell death in the rat prostate following castration.. Prostate1989; 15: 233. Google Scholar 32 : Activation of a Ca2+ - Mg2+-dependent endonuclease as an early event in castration-induced prostatic cell death.. Prostate1988; 13: 103. Google Scholar 33 : Role of calcium in the programmed death of rat prostatic glandular cells.. Prostate1990; 17: 175. Google Scholar 34 : Programmed death of nonproliferating androgen-independent prostatic cancer cells.. Cancer Res.1991; 51: 4693. Google Scholar 35 : Androgen regulation of programmed death of normal and malignant prostatic cells.. J. Androl.1992; 13: 457. Google Scholar From the Department of Urology, University of Bochum, Herne, Germany.© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byMori F, Oda N, Sakuragi M, Sakakibara F, Kiniwa M and Miyoshi K (2008) New Histopathological Experimental Model for Benign Prostatic Hyperplasia: Stromal Hyperplasia in RatsJournal of Urology, VOL. 181, NO. 2, (890-898), Online publication date: 1-Feb-2009.SHARIAT S, ASHFAQ R, ROEHRBORN C, SLAWIN K and LOTAN Y (2018) EXPRESSION OF SURVIVIN AND APOPTOTIC BIOMARKERS IN BENIGN PROSTATIC HYPERPLASIAJournal of Urology, VOL. 174, NO. 5, (2046-2050), Online publication date: 1-Nov-2005.LEE K and PEEHL D (2018) MOLECULAR AND CELLULAR PATHOGENESIS OF BENIGN PROSTATIC HYPERPLASIAJournal of Urology, VOL. 172, NO. 5, (1784-1791), Online publication date: 1-Nov-2004.HEGARTY P, WATSON R, COFFEY R, WEBBER M and FITZPATRICK J (2018) Effects of Cyclic Stretch On Prostatic Cells in CultureJournal of Urology, VOL. 168, NO. 5, (2291-2295), Online publication date: 1-Nov-2002.CHUEH S, GUH J, CHEN J, LAI M and TENG C (2018) DUAL EFFECTS OF OUABAIN ON THE REGULATION OF PROLIFERATION AND APOPTOSIS IN HUMAN PROSTATIC SMOOTH MUSCLE CELLSJournal of Urology, VOL. 166, NO. 1, (347-353), Online publication date: 1-Jul-2001.MARCELLI M, SHAO T, LI X, YIN H, MARANI M, DENNER L, TENG B and CUNNINGHAM G (2018) INDUCTION OF APOPTOSIS IN BPH STROMAL CELLS BY ADENOVIRAL-MEDIATED OVEREXPRESSION OF CASPASE-7Journal of Urology, VOL. 164, NO. 2, (518-525), Online publication date: 1-Aug-2000.DE MARZO A, NELSON W, MEEKER A and COFFEY D (2018) STEM CELL FEATURES OF BENIGN AND MALIGNANT PROSTATE EPITHELIAL CELLSJournal of Urology, VOL. 160, NO. 6 Part 2, (2381-2392), Online publication date: 1-Dec-1998. Volume 158 Issue 1 July 1997 Page: 217-221 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.Metrics Author Information Siegfried Claus More articles by this author Richard Berges More articles by this author Theodor Senge More articles by this author Harald Schulze More articles by this author Expand All Advertisement PDF downloadLoading ...