A New Antifungal Macrolide Component, Brasilinolide B, Produced by Nocardia brasiliensis.
2000; Springer Nature; Volume: 53; Issue: 1 Linguagem: Inglês
10.7164/antibiotics.53.70
ISSN1881-1469
AutoresYuzuru Mikami, Hisayuki Komaki, Tamae Imai, Katsukiyo Yazawa, Akira Nemoto, YASUSHI TANAKA, Udo Graefe,
Tópico(s)Phytochemical compounds biological activities
ResumoOur continuing studies on biologically active compounds from pathogenic microorganism1 2) showed that Nocardia brasiliensis IFM 0466 produces a new antifungal brasilinolide group antibiotic3~5), brasilinolide B (1), distinguishable from brasilinolide A3~5) by its chromatographic properties.This paper describes the fermentation, isolation and structural elucidation of this macrolide (1).The seed broth was prepared by inoculating mycelial elements of the producing strain (N.brasiliensis IFM0466) grown on Sabouraud dextrose agar (Difco) in 10ml brain heart infusion broth with 2%glucose in 50-ml Erlenmeyer shake flasks.The culture was incubated on a rotary shaker at 250rpm for 96 hours.Ten percent inoculum was transferred to a 2.0-liter fermenter containing 1.0 liter of the production mediumcomposed of meat extract 0.5%, peptone 0.5%, glucose 2.0% and supplemented with antifoam 0.05%.The pH was adjusted to 7.4.The jar fermenter was stirred at 500rpmwith aeration at 1.0 liter/minute at 30°C for 4 days.After 4 days of incubation, one and one half volume of methanol was added to the culture broth and further incubated for 3 hours to kill the Nocardia and to extract the active substance from the mycelia.Thereafter the broth was filtered and evaporated under reduced pressure.The crude residue from 10-liter cultures was then subjected to silica gel chromatography using CHCl3-Me0H (1 :0, 10: 1, 5: 1).Active fractions were obtained with CHCL-MeOH(5 : 1) when antifungal pp.70 -74 activity was tested against Aspergillus niger IFM 40406.The combined active fraction was purified by preparative reverse phase HPLC (column; Capcell pal SG120 C18,
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