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Studies on host-virus genome relationship in Epstein-Barr virus immortalized lymphoblastoid cell lines

1986; Academic Press; Volume: 10; Issue: 5 Linguagem: Inglês

10.1016/0309-1651(86)90002-0

1878-240X

Giuseppe Ragona, Domenico Frezza, Alberto Fruscalzo, Richard Jucker, Nicola Rossi, Elena Raimondi, C Barletta, L DECARLI, E. Calef,

Herpesvirus Infections and Treatments

Five human lymphoblastoid cell lines immortalized in vitro with the B95-8 EBV strain, chosen to have a low number of copies of EBV genome, were examined to detect variations in electrophoretic mobility of viral restriction fragments and in the karyotype. Patterns of mobility detected with different viral probes are always the same as those obtained with fragments from purified virus-plasmidic DNA, with one exception. This "non-plasmidic" pattern occurs with a probe containing the termini of the linear virion DNA and consists in an increase of the molecular weight and in the appearance of more than one band. Cytogenetic studies carried on the same cell populations used as source of DNA, early after immortalization, showed a diploid modal chromosome number and no G banding rearrangements.