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Aplysina (Porifera: Demospongiae) species identification through SSCP-ITS patterns

2009; Cambridge University Press; Volume: 90; Issue: 4 Linguagem: Inglês

10.1017/s0025315409991202

1469-7769

Flávia Rachel M. Lamarão, Estéfane Cardinot Reis, T.A. Simão, Rodolpho Mattos Albano, Gisele Lôbo‐Hajdu,

Identification and Quantification in Food

Keratose sponges have no spicules and limited robust morphological characters, causing severe problems for taxonomic classification. Single-strand conformational polymorphism (SSCP) analysis of PCR amplified internal transcribed spacers (ITS) of nuclear ribosomal RNA (rRNA) genes was used to elucidate speciation patterns in marine sponges of the genus Aplysina . In this study, Aplysina species with non-ambiguous traditional morphological alpha taxonomy were used to generate standard SSCP-ITS patterns, which were compared with patterns from alleged new species. Our results show that ITS-SSCP patterns allowed the differentiation of Aplysina sponges, demonstrating the advantage of a method that explores the secondary structure compared to direct sequencing.