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Simultaneous determination of alpha-fetoprotein, human chorionic gonadotropin and estriol in serum of pregnant women by time-resolved fluoroimmunoassay

1999; Elsevier BV; Volume: 20; Issue: 1-2 Linguagem: Inglês

10.1016/s0731-7085(99)00010-2

1873-264X

Katsutoshi Ito, Miwa Oda, Akio Tsuji, Masako Maeda,

Advanced biosensing and bioanalysis techniques

We have developed a simple and rapid time-resolved fluoroimmunoassay (TR-FIA) for simultaneous determination of alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG) and estriol (E3) using europium and samarium ion chelate. In the proposed method, we used a combination of a 96-well microtiter plate for the AFP and hCG assay and transferable solid phase plate for the E3 assay. Therefore, these analytes could be measured simultaneously. The measurable ranges for AFP, hCG and E3 by the proposed method were 3.91–1000 ng ml−1, 877–250 000 IU l−1 and 0.39–100 ng ml−1, respectively. The proposed method which utilized characteristics of a rare earth ion chelate, was convenient (unnecessary diluting samples), quick (96 assays for 2 h), and required only a small quantity sample (50 μl). The principle of this proposed method is applicable to other antigens.