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BIBTEX RIS

Experimental Model of Isolated Lung Perfusion in Rats: First Brazilian Experience Using the IL-2 Isolated Perfused Rat or Guinea Pig Lung System

2010; Elsevier BV; Volume: 42; Issue: 2 Linguagem: Inglês

10.1016/j.transproceed.2010.01.016

1873-2623

Paulo Manuel Pêgo‐Fernandes, Eduardo de Campos Werebe, Paulo Francisco Guerreiro Cardoso, Rogério Pazetti, Karina Andrighetti de Oliveira, Paula Roberta Otaviano Soares, Fábio Biscegli Jatene,

Cardiac Ischemia and Reperfusion

Lung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system. Male Wistar rats weighing 250 g–300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha). Preliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments. The isolated rat lung perfusion system is a reliable method to assess lung preservation.