Site-Specific Pseudouridine Formation in Preribosomal RNA Is Guided by Small Nucleolar RNAs
1997; Cell Press; Volume: 89; Issue: 5 Linguagem: Inglês
10.1016/s0092-8674(00)80263-9
ISSN1097-4172
AutoresPhilippe Ganot, Marie‐Line Bortolin‐Cavaillé, Tamás Kiss,
Tópico(s)Cytomegalovirus and herpesvirus research
ResumoAbstract During the nucleolar maturation of eukaryotic ribosomal RNAs, many selected uridines are converted into pseudouridine by a thus far undefined mechanism. The nucleolus contains a large number of small RNAs (snoRNAs) that share two conserved sequence elements, box H and ACA. In this study, we demonstrate that site-specific pseudouridylation of rRNAs relies on short ribosomal signal sequences that are complementary to sequences in box H/ACA snoRNAs. Genetic depletion and reconstitution studies on yeast snR5 and snR36 snoRNAs demonstrate that box H/ACA snoRNAs function as guide RNAs in rRNA pseudouridylation. These results define a novel function for snoRNAs and further reinforce the idea that base pairing is the most common way to obtain specific substrate–"enzyme" interactions during rRNA maturation.
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