Produção Nacional
Revisado por pares
Anti HSV-1 Activity of Halistanol Sulfate and Halistanol Sulfate C Isolated from Brazilian Marine Sponge Petromica citrina (Demospongiae)
2013; Multidisciplinary Digital Publishing Institute; Volume: 11; Issue: 11 Linguagem: Inglês
10.3390/md11114176
ISSN1660-3397
AutoresTatiana da Rosa Guimarães, Carlos Quiroz, Caroline Rigotto, Simone De Oliveira, Maria De Almeida, Έverson Miguel Bianco, Maria Ester Wollstein Moritz, João Luís Carraro, Jorge A. Palermo, Gabriela M. Cabrera, Eloir Paulo Schenkel, Flávio Henrique Reginatto, Cláudia Maria Oliveira Simões,
Tópico(s)Synthesis and Catalytic Reactions
ResumoThe n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2.
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