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Improvement of the cDNA-AFLP method using fluorescent primers for transcription analysis in bacteria

2005; Elsevier BV; Volume: 63; Issue: 2 Linguagem: Inglês

10.1016/j.mimet.2005.04.002

1872-8359

Francesca Decorosi, Carlo Viti, Alessio Mengoni, Marco Bazzicalupo, Luciana Giovannetti,

Bacteriophages and microbial interactions

Here the cDNA-fluorescent amplified fragment length polymorphism (cDNA-FAFLP) technique, an improvement of cDNA-AFLP method, was used in order to analyse expression profiling in bacteria. The obtained results were validated by real-time PCR. This is the first report that validates the foreseen transcription pattern by cDNA-FAFLP with the application of real-time PCR in bacteria. This new protocol offers the possibility of quick and reliable analysis of transcription profiles, also avoids the problem linked with the use of radioisotopes, and allows a quick identification of genes differentially expressed in bacteria.