Crystallization and Preliminary X-Ray Diffraction Analysis of PD-L1, a Highly Glycosylated Ribosome Inactivating Protein with DNase Activity
2007; Bentham Science Publishers; Volume: 14; Issue: 4 Linguagem: Inglês
10.2174/092986607780363899
ISSN1875-5305
AutoresAlessia Ruggiero, Angela Chambery, Antimo Di Maro, Antonietta Mastroianni, Augusto Parente, Rita Berisio,
Tópico(s)Mycotoxins in Agriculture and Food
ResumoPD-L1 is a highly glycosylated type 1 ribosome inactivating protein, from Phytolacca dioica leaves, with the peculiarity to act also as a DNase. PD-L1 has been successfully crystallized using vapour diffusion and seeding techniques. Crystals belong to the monoclinic C2 space group, with unit cell dimensions a=161.01, b=34.73, c=120.63 Å, β=127.99 . Two molecules are present in the asymmetric unit. Phase determination has been achieved using molecular replacement. Keywords: Ribosome inactivating protein, Phytolacca dioica, crystallization, glycosylation, seeding, X-ray
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