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Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis

2014; National Academy of Sciences; Volume: 111; Issue: 31 Linguagem: Inglês

10.1073/pnas.1404472111

1091-6490

Jonathan Shemesh, Tom Ben‐Arye, Jonathan Avesar, Joo H. Kang, Amir Fine, Michael Super, A. MELLER, Donald E. Ingber, Shulamit Levenberg,

Electrowetting and Microfluidic Technologies

Significance There is a substantial need for single-cell platforms in which each cell is chemically isolated in its own microenvironment and a lack of such platforms that support adherent cells. We present here a method that generates stationary nanoliter droplet arrays on a substrate of choice and supports long-term incubation and interrogation of single cells. We demonstrate the encapsulation of single human dermal fibroblast cells and their substrate attachment, viability, and proliferation, and show they can be retrieved and interfaced to standard cell culturing techniques. We also demonstrate a single-cell metabolic assay and track it over selected groups of indexed traps. The device does not require external equipment or machinery, making it available for point-of-care applications.