Low frequency MFs increased inositol 1,4,5-trisphosphate levels in the Jurkat cell line
1995; Wiley; Volume: 359; Issue: 2-3 Linguagem: Inglês
10.1016/0014-5793(95)00031-4
ISSN1873-3468
AutoresI.L. Korzh-Sleptsova, Erica Lindström, Kjell Hansson Mild, André Berglund, Erik Lundgren,
Tópico(s)Microbial Inactivation Methods
ResumoWe have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T‐cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol ., 156 (1993) 395–398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor molecular mechanisms are at present known. In this study we found that application of a MF to Jurkat cells resulted in significant increase of inositol 1,4,5‐trisphosphate (IP 3 ) levels. Chelation of intracellular calcium ions by BAPTA/AM, did not block the increase of IP 3 induced by MF. This result implied that MF‐induced Ca 2+ oscillations were not due to direct stimulation of the Ca 2+ ‐dependent phospholipase C‐γ1 (PLC‐γ1).
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