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Evaluation of Hydrodynamic Size and Zeta-Potential of Surface-Modified Au Nanoparticle-DNA Conjugates via Ferguson Analysis

2008; American Chemical Society; Volume: 112; Issue: 20 Linguagem: Inglês

10.1021/jp8004426

1932-7455

Sunho Park, Kimberly Hamad‐Schifferli,

Nanopore and Nanochannel Transport Studies

We evaluate Ferguson analysis as a method for simultaneously measuring the size and zeta-potential of gold nanoparticle-DNA (Au NP-DNA) conjugates. This approach is highly suitable when the particle size is <20 nm and biomolecular conformation results in a nonspherical or nonlinear conjugate, causing conventional dynamic light scattering and zeta-potential measurements to be unable to achieve consistent results. We show the applicability of the method by varying the size of gold nanoparticles (5–20 nm) and the loading of methoxypolyethylene glycol/DNA on particle surface and buffer concentrations. Due to the low ionic mobility of tris-borate-EDTA buffer (TBE) and relatively high zeta-potential of Au NP-DNA, Henry's solutions with higher order correction terms are necessary for obtaining the zeta-potential from the measured free mobility and size.