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Influence of Lycopene on Cell Viability, Cell Cycle, and Apoptosis of Human Prostate Cancer and Benign Hyperplastic Cells

2013; Routledge; Volume: 65; Issue: 7 Linguagem: Inglês

10.1080/01635581.2013.812225

1532-7914

Nathalia da Costa Pereira Soares, Anderson Junger Teodoro, Felipe Leite de Oliveira, Carlos Antônio do Nascimento Santos, Christina Maeda Takiya, Oswaldo Saback, Mario Bianco, Antônio Palumbo, Luiz Eurico Nasciutti, Luciana Bueno Ferreira, Etel Rodrigues Pereira Gimba, Radovan Borojević,

Cholesterol and Lipid Metabolism

Prostate cancer is the most common malignancy in men and the second leading cause of cancer-related mortality in men of the Western world. Lycopene has received attention because of its expcted potential to prevent cancer. In the present study, we evaluated the influence of lycopene on cell viability, cell cycle, and apoptosis of human prostate cancer cells and benign prostate hyperplastic cells. Using MTT assay, we observed a decrease of cell viability in all cancer cell lines after treatment with lycopene, which decreased the percentage of cells in G0/G1 phase and increased in S and G2/M phases after 96 h of treatment in metastatic prostate cancer cell lineages. Flow citometry analysis of cell cycle revealed lycopene promoted cell cycle arrest in G0/G1 phase after 48 and 96 h of treatment in a primary cancer cell line. Using real time PCR assay, lycopene also induced apoptosis in prostate cancer cells with altered gene expression of Bax and Bcl-2. No effect was observed in benign prostate hyperplasia cells. These results suggest an effect of lycopene on activity of human prostate cancer cells.