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The influence of tetracyclines on T cell activation

1995; Oxford University Press; Volume: 102; Issue: 3 Linguagem: Inglês

10.1111/j.1365-2249.1995.tb03864.x

1365-2249

M. Kloppenburg, C. L. Verweij, A. M. M. Miltenburg, AJ Verhoeven, M. R. Daha, Ben A. C. Dijkmans, F. C. Breedveld,

Immune Cell Function and Interaction

SUMMARY Minocycline has been shown to have an anti-inflammatory effect in patients with rheumatoid arthritis (RA). Since there is evidence that RA is a T cell-mediated disease, we investigated the effect of minocycline on human T cell clones derived from the synovium of an RA patient. The T cells, when activated via the T cell receptor (TCR)/CD3 complex, were suppressed functionally by minocycline, resulting in a dose-dependent inhibition of T cell proliferation and reduction in production of lL-2. interferon-gamma (IFN-γ) and tumour necrosis faetor-alpha (TNF-α). Besides an inhibition of IL-2 production, mitiocycline exerted its effect on T cell proliferation by induction of a decreased IL-2 responsiveness. We showed that the chelating capacity of minocycline plays a crucial role in the inhibitory effect on T cell function, since the inhibitory effect on T cell proliferation could be annulled by addition of exogenous Ca2+. However, minocycline did not markedly influence the typical TCR/CD3-induced intracellular Ca2+ mobilization. Taken together. the results clearly indicate that minocycline has immunomodulating effects on human T cells.