The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

Artigo Revisado por pares

The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.

1993; Elsevier BV; Volume: 49; Issue: 6 Linguagem: Inglês

10.1016/s0040-4020(01)85819-1

ISSN

1464-5416

Autores

Jordi Marquet, Lourdes Rafecas, Albert Cantos, Marcial Moreno‐Mañas, Maria Cervera, Francisco J. Martínez-Casado, M. Victòria Nogués, Claudi M. Cuchillo,

Tópico(s)

Organic Chemistry Cycloaddition Reactions

Resumo

The photoreactions of 4-nitroveratrole (NVT) and 2-fluoro-4-nitroanisole (FNA) with bovine pancreatic ribonuclease A (RNAase A) and with model nucleophiles are described. From them it is concluded that 2-fluoro-4-nitrophenyl ethers constitute improved alternative biochemical photoprobes, specially for proteins where a number of good nucleophiles are present.

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The Search for Biochemical Photoprobes. III. The Photoreactions of 4-Nitroveratrole and 2-Fluoro-4-nitroanisole with Bovine Pancreatic Ribonuclease A and with Model Nucleophiles.