Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application
1996; Elsevier BV; Volume: 335; Issue: 1-2 Linguagem: Inglês
10.1016/s0003-2670(96)00371-6
ISSN1873-4324
AutoresH. Yanagihara, R. Sakata, H. Minami, Hiroyuki Tanaka, Yukihiro Shoyama, Hiroki Murakami,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoImmunoaffinity column chromatography using anti-forskolin monoclonal antibodies appears to be far superior to previously published separation methods based on crystallization and chromatography. The capacity of the immunoaffinity column as determined by ELISA is 9 μg ml−1. Forskolin has been isolated directly from the crude extracts of tuberous roots and the callus culture of Coleus forskohlii.
Referência(s)