Rapid and accurate high-performance liquid chromatographic method for the determination of 3-methylindole (skatole) in faeces of various species
1991; Elsevier BV; Volume: 566; Issue: 1 Linguagem: Inglês
10.1016/0378-4347(91)80114-r
ISSN1872-812X
AutoresMartin Dehnhard, Hugo Bernal Barragán, R. Claus,
Tópico(s)Meat and Animal Product Quality
ResumoA rapid method for the determination of skatole (3-methylindole) in faeces by reversed-phase high-performance liquid chromatography is described. Samples of 0.5 g were extracted with 2 ml of methanol. The extract was purified on Amberlite XAD-8. The lower limit of detection was 2.5 ng per injection (0.2 μg/g faeces). The mean recovery of skatole was 95%, and the mean coefficients of variation were 7.0% (intra-assay) and 11.8% (inter-assay). Skatole concentrations were clearly lower in faeces from ruminants (average 2.6 μg/g for goat, sheep and cattle) than in those from monogastrics. Mean concentrations in human samples were 15.5 μg/g, and 10 μg/g in mature domestic pigs. An effect of the anabolic status on skatole concentrations in faeces of pigs is likely.
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