The Archaeal Diversity and Population in a Drained Alkaline Saline Soil of the Former Lake Texcoco (Mexico)
2011; Taylor & Francis; Volume: 29; Issue: 1 Linguagem: Inglês
10.1080/01490451.2010.520075
ISSN1521-0529
AutoresCésar Valenzuela-Encinas, Rocío Alcántara‐Hernández, María Isabel Estrada‐Alvarado, Francisco Javier Zavala-Díaz de la Serna, Luc Dendooven, Rodolfo Marsch,
Tópico(s)Methane Hydrates and Related Phenomena
ResumoDraining soil of the former Lake Texcoco, Mexico with pH > 10.0 and electrolytic conductivity (EC) > 100 dS m−1 for 17 years has reduced pH to 7.8 and EC to 0.68 dS m−1. Metagenomic DNA from the archaeal community was extracted directly from this soil and used as template to amplify the 16S ribosomal genes by PCR to construct gene libraries. Most of the cloned Archaea were related to mesophilic crenarchaeota and were not-yet-cultured. Sequence and phylogenetic analyses of these clones identified a group of Archaea with close affiliation to the ammonia-oxidizing Archaea. The cloned sequences from the drained soil diverged clearly from Haloarchaea found in the undrained soil from the lake.
Referência(s)