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Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves

1999; Oxford University Press; Volume: 180; Issue: 2 Linguagem: Inglês

10.1111/j.1574-6968.1999.tb08794.x

1574-6968

Philippe Berto, Pascal Comménil, Lionel Belingheri, Bertrand Dehorter,

Transgenic Plants and Applications

Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted with Botrytis cinerea anti-lipase antibodies, which were reported to inhibit the in vitro lipase activity. Anti-lipase antibodies were added to a conidial suspension of A. brassicicola prior to inoculation. As a result, blackspot lesions were reduced by 90% on intact cauliflower leaves, but not on leaves from which surface wax had been removed. Spore surface-bound lipase is thought to interact closely with epicuticular leaf waxes for adhesion and/or penetration of the fungal propagules during the early stages of host-parasite interactions.