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Fluorescence study of N-(3-pyrene)maleimide conjugated to rabbit skeletal F-actin and plasmodium actin polymers

1976; Elsevier BV; Volume: 446; Issue: 1 Linguagem: Inglês

10.1016/0005-2795(76)90108-2

1878-1454

Yoshihiro Kawasaki, Koshin Mihashi, Hideyuki Tanaka, H. Ohnuma,

3D Printing in Biomedical Research

A fluorescent probe N-(3-pyrene)maleimide was conjugated to rabbit skeletal F-actin at the site of most reactive sulfhydryl group (Cys-373). Its fluorescence anisotropy decay showed a single correlation time of 560 ns at 25 °C, which is in a very good agreement with the correlation time of the dansyl-l-cysteine group conjugated to the same site of F-actin reported very recently [Wahl, Ph., Mihashi, K. and Auchet, J-C. (1975) FEBS Lett. 8, 164–167]. Actin from plasmodia of myxomycates, Physarum polycepharum, was also conjugated with N-(3-pyrene) maleimide and the fluorescence anisotropy was compared with rabbit skeletal F-actin using the classical steady excitation method. It was found that the internal mobility of the magnesium polymer of plasmodium actin is remarkably larger than both plasmodium F-actin and rabbit skeletal F-actin.