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Asymmetric synthesis of chiral cyclic amine from cyclic imine by bacterial whole-cell catalyst of enantioselective imine reductase

2010; Royal Society of Chemistry; Volume: 8; Issue: 20 Linguagem: Inglês

10.1039/c0ob00353k

1477-0539

Koichi Mitsukura, Mai Suzuki, Kazuhiro Tada, Toyokazu Yoshida, Tôru Nagasawa,

Amino Acid Enzymes and Metabolism

Streptomyces sp. GF3587 and 3546 were found to be imine-reducing strains with high R- and S-selectivity by screening using 2-methyl-1-pyrroline (2-MPN). Their whole-cell catalysts produced 91 mM R-2-methylpyrrolidine (R-2-MP) with 99.2%e.e. and 27.5 mM S-2-MP (92.3%e.e.) from 2-MPN at 91-92% conversion in the presence of glucose, respectively.