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The Urokinase Plasminogen Activator Receptor–Associated Protein/Endo180 Is Coexpressed with Its Interaction Partners Urokinase Plasminogen Activator Receptor and Matrix Metalloprotease-13 during Osteogenesis

2001; Elsevier BV; Volume: 81; Issue: 10 Linguagem: Inglês

10.1038/labinvest.3780354

1530-0307

Lars H. Engelholm, Boye Schnack Nielsen, Sarah Netzel–Arnett, Helene Solberg, Xiao‐Dong Chen, José M. López, Carlos López-Otı́n, Marian F. Young, Henning Birkedal‐Hansen, Keld Danø, Leif R. Lund, Niels Behrendt, Thomas Bugge,

Bone and Dental Protein Studies

The urokinase plasminogen activator receptor–associated protein/Endo180 (uPARAP/Endo180) is a newly discovered member of the macrophage mannose receptor family that was reported to interact with ligand-bound urokinase plasminogen activator receptor (uPAR), matrix metalloprotease-13 (MMP-13), and collagen V on the cell surface. We have determined the sites of expression of this novel receptor during murine postimplantation development. uPARAP/Endo180 was expressed in all tissues undergoing primary ossification, including the developing bones of the viscerocranium and calvarium that ossify intramembranously, and developing long bones undergoing endochondral ossification. uPARAP/Endo180 mRNA was expressed by both immature osteoblasts and by mature osteocalcin-producing osteoblasts-osteocytes, and was coexpressed with MMP-13. Interestingly, osteoblasts also expressed uPAR. Besides bone-forming tissues, uPARAP/Endo180 expression was detected only in a mesenchymal condensation of the midbrain and in the developing lungs. The data suggest a function of this novel protease receptor in bone development, possibly mediated through its interactions with uPAR, MMP-13, or collagen V.