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Caspase-1 is active since the early phase of rheumatoid arthritis

2011; BMJ; Volume: 70; Issue: Suppl 2 Linguagem: Inglês

10.1136/ard.2010.149096.4

1468-2060

Rita Cascão, Joaquim Polido‐Pereira, Helena Canhão, Ana Maria Rodrigues, Márcio Navalho, Helena Raquel, AF Mourão, Cristina Maria Mendes Resende, João Eurico Fonseca, Luís F. Moita,

Rheumatoid Arthritis Research and Therapies

Background We have previously reported an increase in interleukin (IL)-1β levels in very recent onset arthritis. Therefore, molecular mechanisms that regulate IL-1β secretion, such as caspase-1 activation, might play a relevant role in arthritis onset and progression. Objectives Our main goal was to evaluate caspase-1 state of activation in early polyarthritis and established rheumatoid arthritis (RA) patients. Materials and methods Blood samples were collected from 12 untreated early polyarthritis patients, 12 established RA patients and 10 healthy controls. Caspase-1 activity was assessed using a carboxyfluorescein FLICA detection kit. Results We found that both early polyarthritis and established RA patients have higher active caspase-1 levels. Surprisingly, caspase-1 activation levels in patient cells remain unchanged when cells were stimulated with Escherichia coli and ATP, whereas in control cells caspase-1 levels increased after stimulation. We observed no significant differences when comparing early polyarthritis with established RA samples. However, in RA patients with higher disease activity the proportion of active caspase-1 is higher. Conclusions Our results suggest that leucocytes in both early polyarthritis and established RA patients have high baseline levels of activated caspase-1, which cannot be significantly increased after stimulation. Altogether, our data supports the hypothesis that caspase-1 is activated in early arthritis.