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Vitamin D receptor stable transfection restores the susceptibility to 1,25-dihydroxyvitamin D3cytotoxicity in a rat glioma resistant clone

1998; Wiley; Volume: 52; Issue: 2 Linguagem: Inglês

10.1002/(sici)1097-4547(19980415)52

1097-4547

Nathalie Davoust, Didier Wion, Guillemette Chevalier, M Garabédian, Philippe Brachet, Dominique Couez,

Estrogen and related hormone effects

Journal of Neuroscience ResearchVolume 52, Issue 2 p. 210-219 Vitamin D receptor stable transfection restores the susceptibility to 1,25-dihydroxyvitamin D3cytotoxicity in a rat glioma resistant clone Nathalie Davoust, Nathalie Davoust Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorDidier Wion, Didier Wion Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorGuillemette Chevalier, Guillemette Chevalier Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorMichèle Garabedian, Michèle Garabedian Centre National de la Recherche Scientifique, Hopital Saint-Vincent-de-Paul, Paris, FranceSearch for more papers by this authorPhilippe Brachet, Philippe Brachet Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorDominique Couez, Corresponding Author Dominique Couez [email protected] Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceINSERM U298, CHU, F-49033 Angers Cedex 01, FranceSearch for more papers by this author Nathalie Davoust, Nathalie Davoust Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorDidier Wion, Didier Wion Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorGuillemette Chevalier, Guillemette Chevalier Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorMichèle Garabedian, Michèle Garabedian Centre National de la Recherche Scientifique, Hopital Saint-Vincent-de-Paul, Paris, FranceSearch for more papers by this authorPhilippe Brachet, Philippe Brachet Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceSearch for more papers by this authorDominique Couez, Corresponding Author Dominique Couez [email protected] Institut National de la Santé et de la Recherche Médicale, Centre Hospitalier Universitaire, Angers, FranceINSERM U298, CHU, F-49033 Angers Cedex 01, FranceSearch for more papers by this author First published: 07 December 1998 https://doi.org/10.1002/(SICI)1097-4547(19980415)52:2 3.0.CO;2-DCitations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Recently, 1,25-dihydroxyvitamin D3 (1,25-D3) and less hypercalcemic analogs were shown to exert a delayed cytotoxic effect on rat C6 glioma cells. 1,25-D3 induces in these cells a programmed cell death, accompanied by the induction of c-myc, p53 and gadd 45 genes. The involvement of the intracellular vitamin D receptor (VDR) remained to be determined. In this lethal process, we have investigated its role in a subclone of C6 cells, which was isolated on the basis of its resistance to 1,25-D3, and in which VDR expression was not detected either at the mRNA or protein levels. The stable transfection of a rat VDR cDNA into this clone restored its susceptibility to the cytotoxic effects of 1,25-D3. This phenomenon was accompanied by a dramatic upregulation of c-myc mRNA expression, as already described in a C6-sensitive clone. These results provide the first evidence that VDR expression, if not sufficient, is necessary to mediate 1,25-D3 cytotoxic effect in C6 glioma cells. Since VDR mRNA expression has been already reported in human brain tumors, our data imply that the identification of VDR expression could become a prerequisite in any strategy of glioma treatment with vitamin D analogs. J. Neurosci. Res. 52:210–219, 1998. © 1998 Wiley-Liss, Inc. References Auffray C, Rougeon F (1980): Purification of mouse immunoglobulin heavy chain messenger RNAs for total myeloma tumor RNA. Eur J Biochem 107: 303– 314. Baudet C, Chevalier G, Naveilhan P, Binderup L, Brachet P, Wion D (1996a): Cytotoxic effects of 1α,25-dihydroxyvitamin D3 and synthetic vitamin D3 analogues on a glioma cell line. Cancer Lett 100: 3– 10. Baudet C, Chevalier G, Chassevent A, Canova C, Filmon R, Larra F, Brachet P, Wion D (1996b): 1,25-dihydroxyvitamin D3 induces programmed cell death in a rat glioma cell line. J Neurosci Res 46: 540– 550. Boland R, de Boland A, Vasquez G, Santillan G, Monje P (1997): Molecular aspects of rapid responses to 1,25(OH)2-Vitamin D3. In A Norman, R Bouillon, M Thomasset (eds): ' Vitamin D. Chemistry, Biology and Clinical Applications of the Steroid Hormone.' Riverside: University of California, pp 361– 368. Burmester JK, Wiese RJ, Maeda N, DeLuca HF (1988): Structure and regulation of the rat 1,25-dihydroxyvitamin D3 receptor. Proc Natl Acad Sci USA 85: 9499– 9502. Carlberg C, Bendik I, Wyss A, Meier E, Sturzenbecker LJ, Grippo J, Hunziker W (1993): Two nuclear signalling pathways for vitamin D. Nature 361: 657– 660. Christakos S, Raval-Pandya M, Werny RP, Yang W (1996): Genomic mechanisms involved in the pleiotropic actions of 1,25-dihydroxyvitamin D3. Biochem J 316: 361– 371. Couez D, Pagès F, Raguenau M, Nunès J, Klassen S, Mavas C, Truneh A, Olive D (1994): Functional expression of human CD28 in murine T cell hybridomas. Mol Immunol 31: 47– 57. Cross HS, Bajna E, Bises G, Genser D, Kállay E, Pötzi R, Wenli E, Wrba F, Roka R, Peterlik M (1996): Vitamin D receptor and cytokeratin expression may be progression indicators in human colon cancer. Anticancer Res 16: 2333– 2338. Evan GI, Willie AH, Gilbert CS, Littlewood TD, Land H, Brooks M, Waters CM, Penn LZ, Hancock DC (1992): Induction of apoptosis in fibroblasts by c-myc protein. Cell 69: 119– 128. Freedman L, Anderson E, Bromleigh V, Cheskis B, Lemon B, Liu M, Rachez C, Rots N, Suldan Z, Towers T (1997): Transcriptional targets of the vitamin D3 receptor mediating cell cycle arrest and differentiation. In A Norman, R Bouillon, M Thomasset (eds): ' Vitamin D. Chemistry, Biology and Clinical Applications of the Steroid Hormone.' Riverside: University of California, pp 188– 194. Garcion E, Nataf S, Berod A, Darcy F, Brachet P (1997): 1,25-Dihydroxyvitamin D3 inhibits the expression of inducible nitric oxide synthase in rat central nervous system during experimental allergic encephalomyelitis. Mol Brain Res 45: 255– 267. Gunning P, Leavitt J, Muscat G, Ng SY, Kedes L (1987): A human β-actin expression vector directs high-level accumulation of antisense transcripts. Proc Natl Acad Sci USA 84: 4831– 4835. Haussler M, Jurutka P, Haussler C, Hsieh J, Thompson P, Remus L, Selznick S, Encinas C, Whitfield K (1997): VDR-mediated transactivation: interplay between 1,25(OH)2D3, RXR heterodimerization, transcription (co) factors and polymorphic receptor variants. In A Norman, R Bouillon, M Thomasset (eds): ' Vitamin D. Chemistry, Biology and Clinical Applications of the Steroid Hormone.' Riverside: University of California, pp 210– 217. Hedlund E, Moffatt KA, Miller GJ (1996): Stable expression of the nuclear vitamin D receptor in the human prostatic carcinoma cell line JCA-1: Evidence that the antiproliferative effects of lα,25-dihydroxyvitamin D3 are mediated exclusively through the genomic signaling pathway. Endocrinology 137: 1554– 1561. Hewison M, Dabrowski L, Faulker L, Hughson E, Vadher S, Rut AR, Brickell PM, O'Riordan JLH, Katz DR (1994): Transfection of vitamin D receptor cDNA into the monoblastoid cell line U 937: The role of vitamine D3 in homotypic macrophage adhesion. J Immunol 153: 5709– 5719. Jordan JP, Hand CM, Markowitz RS, Black P (1992): Test for chemotherapeutic sensitivity of cerebral gliomas: Use of colorimetric MTT assay. J Neuro-oncol 14: 19– 35. Kaiser U, Schilli M, Wegmann B, Barth P, Wedel S, Hofmann J, Havemann K (1996): Expression of vitamin D receptor in lung cancer. J Cancer Res Clin Oncol 122: 356– 359. Le Mellay V, Grosse B, Lieberherr M (1997): Phospholipase C beta and membrane action of calcitriol and estradiol. J Biol Chem 272: 11902– 11907. Lemon BD, Freedman LP (1996): Selective effects of ligands on vitamin D3 receptor and retinoid X receptor-mediated gene activation in vivo. Mol Cel Biol 16: 1006– 1016. Magrassi L, Bono F, Milanesi G, Butti G (1992): Vitamin D receptor expression in human brain tumors. J Neurosurg Sci 36: 27– 30. Magrassi L, Butti G, Pezzotta S, Infuso I, Milanesi G (1995): Effects of vitamin D and retinoic acid on human glioblastoma cell lines. Act Neurochir (Wien) 133: 184– 190. Maniatis T, Fritsch EF, Sambrook J (1982): ' Molecular Cloning. A Laboratory Manual.' Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. Manolagas SC, Hustmyer FG, Yu XP (1990): Immunomodulating properties of 1,25-dihydroxyvitamin D3. Kidney Int 38: 9– 16. Morimoto S, Yoshikawa K, Kozuka T (1986): An open study of vitamin D3 treatment in psoriasis. Br J Dermatol 115: 421– 429. Narvaez CJ, Vanweelden K, Byrne I, Welsh JE (1996): Characterization of a vitamin D3-resistant MCF-7 cell line. Endocrinology 137: 400– 409. Nataf S, Garcion E, Darcy F, Brachet P (1996): 1,25-Dihydroxyvitamin D3 exerts regional effects in the central nervous system during experimental allergic encephalomyelitis. J Neuropathol Exp Neurol 55: 904– 914. Naveilhan P, Neveu I, Baudet C, Ohyama KY, Brachet P, Wion D (1993): Expression of 25(OH) vitamin D3 24-hydroxylase gene in glial cells. NeuroReport 5: 255– 257. Naveilhan P, Berger F, Haddad K, Barbot N, Benabid AL, Brachet P, Wion D (1994): Induction of glioma cell death by 1,25(OH)2 vitamin D 3: Towards an endocrine therapy of brain tumors. J Neurosci Res 37: 271– 277. Neveu I, Naveilhan P, Jehan F, Baudet C, Wion D, DeLuca HF, Brachet P (1994a): 1,25-dihydroxyvitamin D3 regulates the synthesis of nerve growth factor in primary cultures of glial cells. Mol Brain Res 24: 70– 76. Neveu I, Naveilhan P, Baudet C, Brachet P, Metsis M (1994b): 1,25-dihydroxyvitamin D3 regulates NT-3, NT-4 but not BDNF mRNA in astrocytes. Neuroreport 6: 124– 126. Neveu I, Naveillan P, Menaa C, Wion D, Brachet P, Garabedian M (1994c): Synthesis of 1,25-dihydroxyvitamin D3 by rat brain macrophages in vitro. J Neurosci Res 38: 214– 220. Norman AW (1995): The vitamin D endocrine system: Manipulation of structure-function relationships to provide opportunities for development of new cancer chemopreventive and immunosuppressive agents. J Cell Biochem 22: 218– 225. Röser K, Bohn W, Giese G, Mannweiler K (1991): Subclones of C6 rat glioma cells differing in intermediate filament protein expression. Exp Cell Res 197: 200– 206. Schräder M, Müller, KM, Carlberg C (1994): Specificity and flexibility of vitamin D signaling. J Biol Chem 269: 5501– 5504. Shabahang M, Buras R, Davoodi F, Schumaker L, Nauta R, Evans S (1993): 1,25-Dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differenciation and growth inhibition. Cancer Res 53: 3712– 3719. Shabahang M, Buffan A, Nolla J, Schumaker L, Brenner R, Buras R, Nauta R, Evans S (1996): The effect of 1,25-dihydroxyvitamin D3 on the growth of soft-tissue sarcoma cells as mediated by the vitamin D receptor. Ann Surg Oncol 3: 144– 149. Skowronski RJ, Peehl DM, Feldman D (1993): Vitamin D and prostate cancer: 1,25-dihydroxyvitamin D3 receptors and actions in human prostate cancer cell lines. Endocrinology 132: 1952– 1960. Smith CA, Williams GT, Kingston R, Jenkinson EJ, Owen JT (1989): Antibodies to CD3/T-cell receptor induce death by apoptosis in immature T cells in thymic cultures. Nature 337: 181– 184. Studzinski GP, McLane JA, Uskokovic MR (1993): Signaling pathways for vitamin D-induced differentiation: Implication for therapy of proliferative and neoplastic diseases. Crit Rev Eukaryote Gene Express 3: 279– 312. Stumpf WE, Madhabananda S, Clark SA (1982): Brain target sites for 1,25-dihydroxyvitamine D3. Science 215: 1403– 1405. Stumpf WE, O'Brien LP (1987): 1,25-(OH)2 Vitamin D3 sites of action in the brain: An autoradiography study. Histochemistry 87: 393– 406. Sutherland MK, Somerville MJ, Yoong LKK, Bergeron C, Haussler MR, Mc Lachlan DRC (1992): Reduction of vitamin D hormone receptor mRNA levels in Alzheimer as compared to Huntington hippocampus: Correlation with calbindin-28k mRNA levels. Mol Brain Res 19: 239– 250. Wecksler WR, Norman AW (1979): An hydroxylapatite batch assay for the quantitation of 1,25-dihydroxyvitamin D3 receptor complexes. Anal Biochem 92: 314– 323. Welsh JE (1994): Induction of apoptosis in breast cancer cells in response to vitamine D and antiestrogens. Biochem Cell Biol 72: 537– 545. Citing Literature Volume52, Issue215 April 1998Pages 210-219 ReferencesRelatedInformation