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PCR‐RFLP is as sensitive and reliable as PCR‐SSO in HLA class II genotyping

1992; Wiley; Volume: 40; Issue: 2 Linguagem: Inglês

10.1111/j.1399-0039.1992.tb01967.x

1399-0039

Nobuhisa Mizuki, Shigeaki Ohno, Kazuhito Sugimura, Takeshi Seki, Yukie Y. Kikuti, Asako Ando, Masao Ôta, Kimiyoshi Tsuji, Hidetoshi Inoko,

Immunodeficiency and Autoimmune Disorders

Tissue AntigensVolume 40, Issue 2 p. 100-103 PCR-RFLP is as sensitive and reliable as PCR-SSO in HLA class II genotyping Nobuhisa Mizuki, Nobuhisa Mizuki Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, KanagawaSearch for more papers by this authorShigeaki Ohno, Shigeaki Ohno Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, KanagawaSearch for more papers by this authorKazuhito Sugimura, Kazuhito Sugimura Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorTakeshi Seki, Takeshi Seki Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorYukie Yara Kikuti, Yukie Yara Kikuti Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorAsako Ando, Asako Ando Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMasao Ota, Masao Ota Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorKimiyoshi Tsuji, Kimiyoshi Tsuji Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorHidetoshi Inoko, Corresponding Author Hidetoshi Inoko Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanAddress: Dr. Hidetoshi Inoko Department of Molecular Life Science Tokai University School of Medicine Bohseidai, Isehara, 259–11 Japan: Fax: 463–92–4750Search for more papers by this author Nobuhisa Mizuki, Nobuhisa Mizuki Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, KanagawaSearch for more papers by this authorShigeaki Ohno, Shigeaki Ohno Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, KanagawaSearch for more papers by this authorKazuhito Sugimura, Kazuhito Sugimura Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorTakeshi Seki, Takeshi Seki Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorYukie Yara Kikuti, Yukie Yara Kikuti Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorAsako Ando, Asako Ando Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorMasao Ota, Masao Ota Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorKimiyoshi Tsuji, Kimiyoshi Tsuji Department of Transplantation, Tokai University School of Medicine, Isehara, Kanagawa, JapanSearch for more papers by this authorHidetoshi Inoko, Corresponding Author Hidetoshi Inoko Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, JapanAddress: Dr. Hidetoshi Inoko Department of Molecular Life Science Tokai University School of Medicine Bohseidai, Isehara, 259–11 Japan: Fax: 463–92–4750Search for more papers by this author First published: August 1992 https://doi.org/10.1111/j.1399-0039.1992.tb01967.xCitations: 21AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 Higuchi R. Simple and rapid preparation of samples for PCR. In: HA Erlich, ed. PCR Technology. New York: The Stockton Press, 1989: 31–38. 2 Bugawan TL, Begovich AB, Erlich HA. Rapid HLA-DPB typing using enzymatically amplified DNA and nonradioactive sequence-specific oligonucleotide probes. Immunogenetics 1990: 32: 231–41. 3 Maeda M, Murayama N, Ishii H, et al. A simple and rapid method for HLA-DQA1 genotyping by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 1989: 34: 290–8. 4 Uryu N, Maeda M, Ota M, Tsuji K, Inoko H. A simple and rapid method for HLA-DRB and -DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 1990: 35: 20–31. 5 Maeda M, Uryu N, Murayama N, et al. A simple and rapid method for HLA-DP genotyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases. Hum Immunol 1990: 27: 111–21. 6 Inoko H. PCR-RFLP method holds great promise for complete HLA class II genotyping. Tissue Antigens 1990: 23: 88–92. 7 Nomura N, Ota M, Tsuji K, Inoko H. HLA-DQB1 genotyping by modified PCR-RFLP method combined with allele-specific primers. Tissue Antigens 1991: 38: 53–59. 8 Ota M, Seki T, Nomura N, et al. Modified PCR-RFLP method for HLA-DPB1 and -DQA1 genotyping. Tissue Antigens 1991: 38: 60–71. 9 Ota M, Seki T, Fukushima H, Tsuji K, Inoko H. HLA-DRB1 genotyping by modified PCR-RFLP method combined with group-specific primers. Tissue Antigens (in press). 10 Dekker JW, Easteal S. HLA-DP typing by amplified fragment length polymorphisms (RFLPs). Immunogenetics 1990: 32: 56–59. 11 Yunis I, Salazar M, Yunis EJ. HLA-DR generic typing by RFLP. Tissue Antigens 1991: 38: 78–88. 12 Ju LY, Gu XF, Bardie R, Krishnamoorthy R, Charron D. A simple nonradioactive method of DNA typing for subsets of HLA-DR4: prevalence data on HLA-DR4 subsets in three diabetic population groups. Hum Immunol 1991: 31: 251–8. Citing Literature Volume40, Issue2August 1992Pages 100-103 ReferencesRelatedInformation