Polymerase chain reaction engineering
1997; Wiley; Volume: 55; Issue: 2 Linguagem: Inglês
10.1002/(sici)1097-0290(19970720)55
ISSN1097-0290
AutoresJames T. Hsu, Simantini Das, Satish Mohapatra,
Tópico(s)Molecular Biology Techniques and Applications
ResumoA mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the amplification of DNA. Computer simulation of the model is carried out to determine the effects of various parameters, such as the cycle number, initial DNA concentration (copynumber), initial enzyme concentration, extension time, temperature ramp, and enzyme deactivation on the DNA generation. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 359-366, 1997.
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