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A Double-Injection Technique for in vivo Measurement of Dopamine D2-Receptor Density in Monkeys with 3-(2'-[ 18 F] Fluoroethyl)Spiperone and Dynamic Positron Emission Tomography

1989; SAGE Publishing; Volume: 9; Issue: 6 Linguagem: Inglês

10.1038/jcbfm.1989.119

1559-7016

Sung‐Cheng Huang, Mark M. Bahn, Jorge R. Barrio, John M. Hoffman, Nagichettiar Satyamurthy, Randall A. Hawkins, John C. Mazziotta, Michael E. Phelps,

Neuroscience and Neuropharmacology Research

Dopamine D2-receptor density in striatum of monkey was measured with 3-(2'-[ 18 F]fluoroethyl)spiperone (FESP) and dynamic positron emission tomography (PET), using a double-injection technique. A first bolus of high specific activity (SA) FESP (5 mCi; ≃ 1 Ci/μmol) was injected i.v.; 90 min later, a second bolus of lower SA FESP (5 mCi; ≃ 0.04 Ci/μmol) was injected. A dynamic PET study was performed to measure the kinetics of FESP in striatum over 180 min, and the metabolite-corrected concentration of FESP in plasma as a function of time was obtained from arterial blood samples. A nonlinear compartmental model that took into account the saturability of the receptor binding was used to describe the kinetics of FESP in striatum. Model parameters were estimated by regression with a constraint based on information about the equilibrium dissociation constant of the ligand–receptor binding. Dopamine D2-receptor density in striatum was estimated to be 25.9 ± 12.7 pmol/g in seven Macaca nemestrina monkeys. The method does not require the use of cerebellum as a reference tissue region and an estimate of dopamine D2-receptor density can be obtained from a single study.