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On the determination of available lysine in casein and rapeseed protein concentrates using 2,4,6-trinitrobenzenesulphonic acid (TNBS) as a reagent for free epsilon amino group of lysine

1976; Elsevier BV; Volume: 70; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(76)90467-x

1096-0309

Anders Eklund,

Probiotics and Fermented Foods

The determination of available lysine was studied using 2,4,6-trinitrobenzene-sulfonic acid (TNBS) as a reagent for free epsilon amino group of lysine. By establishing optimal conditions for the hydrolysis of TNP-labelled protein, a modification of a previously described TNBS-method was proposed. The changes reduced the analytical variations substantially from the original method and made it possible to obtain in a simple and rapid way quite reproducible values for the contents of available lysine in casein and rapeseed protein concentrates.