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On the Contents of the Central Vacuole in the <i>Mimosa</i> Motor Cell

1971; International Society of Cytology; Volume: 36; Issue: 2 Linguagem: Inglês

10.1508/cytologia.36.359

1348-7019

Hideo Toriyama, Syôiti Satô,

Lipid Membrane Structure and Behavior

Optical microscopy: It is possible to distinguish the stimulated Mimosa motor cell from the non-stimulated one, based on fixation with Müller's fluid followed by methyl blue staining. The shrinkage of the protoplast is clearly visible after stimulation. Under these conditions the contents of the central vacuole can not be markedly discriminated from the protoplasm. By treatment with Weber-Edsall solution the contents of the central vacuole disappear. While, in the same condition the protoplasm remains at the periphery of the motor cell. By this technique, it is made possible to distinguish the contents of central vacuole from the protoplasm. There are scarcely any contents of the central vacuole observable in some cells, while the contents are more or less clearly visible in other cells. At any rate, it is suggestive of the contents of the vacuole in the motor cell dissolved with Weber-Edsall solution.Electron microscopy: Before stimulation, diffused fine fibrillar contents of the central vacuole are clearly observable; they are 20-30mμ thick and 200-400mμ long. Upon stimulation, however the contents of the central vacuole change in appearance, becoming shrunken and aggregated pieces.