Portal de Periódicos da CAPES

Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya.

1997; Polish Biochemical Society; Volume: 44; Issue: 3 Linguagem: Inglês

10.18388/abp.1997_4408

1734-154X

Maciej Kozak, E Kozian, Zbigniew Grzonka, Mariusz Jaskólski,

Phytochemistry and biological activities of Ficus species

A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crystals are monoclinic, space group P2(1), with unit cell parameters a = 42.6 A, b = 49.8 A, c = 50.5 A, A = 111.9 degrees, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 A. A molecular model has been placed in the unit cell by molecular replacement.