CD4-Independent Binding of SIV gp120 to Rhesus CCR5
1997; American Association for the Advancement of Science; Volume: 278; Issue: 5342 Linguagem: Inglês
10.1126/science.278.5342.1470
ISSN1095-9203
AutoresKathleen A. Martin, Richard T. Wyatt, Michael Farzan, Hyeryun Choe, Luisa Marcon, Elizabeth Desjardins, James E. Robinson, Joseph Sodroski, Craig Gérard, Norma P. Gerard,
Tópico(s)HIV/AIDS drug development and treatment
ResumoCCR5 and CD4 are coreceptors for immunodeficiency virus entry into target cells. The gp120 envelope glycoprotein from human immunodeficiency virus strain HIV-1(YU2) bound human CCR5 (CCR5 hu ) or rhesus macaque CCR5 (CCR5 rh ) only in the presence of CD4. The gp120 from simian immunodeficiency virus strain SIVmac239 bound CCR5 rh without CD4, but CCR5 hu remained CD4-dependent. The CD4-independent binding of SIVmac239 gp120 depended on a single amino acid, Asp 13 , in the CCR5 rh amino-terminus. Thus, CCR5-binding moieties on the immunodeficiency virus envelope glycoprotein can be generated by interaction with CD4 or by direct interaction with the CCR5 amino-terminus. These results may have implications for the evolution of receptor use among lentiviruses as well as utility in the development of effective intervention.
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