Portal de Periódicos da CAPES

Flower Colour Breaking in Two Aubrieta Cultivars Caused by Cucumber Mosaic Cucumovirus (CMV) Lacking Satellite RNA-5

2001; Akadémiai Kiadó; Volume: 36; Issue: 3-4 Linguagem: Inglês

10.1556/aphyt.36.2001.3-4.15

1588-2691

J Horváth, Mladen Krajačić, Đorđe Mamula,

Plant Disease Resistance and Genetics

Cucumber mosaic cucumovirus (CMV), isolate Aub (CMV-Aub), was the only virus found in plants of Aubrieta cvs Blue Emperor and Gloriosa showing symptom of flower colour breaking. CMV-characteristic electrophoretic banding pattern of replicative RNA (dsRNA), lacking satellite RNA-5 (CARNA5), was easily recognized, especially when the RNA was purified from experimentally infected Nicotiana glutinosa L. and N. megalosiphon Heurck et Muell. plants, but also from original hosts. The pattern was absent in symptom-free Aubrieta plants. Virus identification was supported, in addition, by test plant and serological reactions. CMV-Aub is a rather unstable isolate; its particles were detected in purified preparations before and after density gradient centrifugation, however, only in fixed preparations (1% formaldehyde). The virus induced mild leaf symptoms in Cucumis sativus L. cv. Delicatess. No difference was found between virus isolates from the two Aubrieta cultivars. There were also indications of virus presence in Aubrieta cvs Gartenstadt Purpur and Vesuv.