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Endothelin-1 as a Putative Modulator of Erectile Dysfunction: I. Characteristics of Contraction of Isolated Corporal Tissue Strips

1995; Lippincott Williams & Wilkins; Volume: 153; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(01)67390-7

1527-3792

George J. Christ, Seth E. Lerner, Daniel C. Kim, Arnold Melman,

Urinary Bladder and Prostate Research

No AccessJournal of UrologyInvestigative Urology1 Jun 1995Endothelin-1 as a Putative Modulator of Erectile Dysfunction: I. Characteristics of Contraction of Isolated Corporal Tissue Strips George J. Christ, Seth E. Lerner, Daniel C. Kim, and Arnold Melman George J. ChristGeorge J. Christ , Seth E. LernerSeth E. Lerner , Daniel C. KimDaniel C. Kim , and Arnold MelmanArnold Melman View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)67390-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Normal erectile physiology is heavily dependent on a delicate balance between the effects of endogenous vasoconstricting and vasorelaxing hormones on the tone of the corporal smooth muscle. Recent studies indicate that endothelin-1 (ET-1) is present and physiologically active in the human corpora. The primary goal of the present investigation was to further define the role of ET-1 in corporal physiology and to ascertain whether it might play a role in augmenting corporal tone in vivo, as reported in other vascular tissues. Thus, we conducted pharmacological studies of ET-1-induced steady-state contractions in isolated human corporal smooth muscle strips to determine if there were any detectable age- or diabetes-related alterations in ET-1-induced contractions. For statistical analysis, the patient population was divided into 2 age groups, A (less or equal to 59 years of age; = 11 patients) and B (greater or equal to 60 years of age; n = 7 patients), and further subdivided into 2 diagnostic categories, diabetic (n = 7 patients) and nondiabetic (n = 11 patients). Construction of cumulative concentration response curves (CRCs) for ET-1-induced contractions revealed characteristically slow onset and long-lasting responses. Endothelin-1 CRC data were computer fit to the logistic equation to derive Emax (calculated maximal response), pEC50 (negative logarithm of the concentration that elicits one-half of the calculated maximal response) and slope factor (n) values. Two-factor analysis of variance revealed no detectable age- or diabetes-related alterations, nor any age-diabetes interaction in any of the logistic parameters. Furthermore, logistic analysis of ET-1 CRC data on 14 isolated corporal tissue strips derived from 3 potent patients with documented spontaneous erections revealed no differences in ET-1 contractility from that observed for patients with organic erectile dysfunction. 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Google Scholar Department of Urology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, New York© 1995 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byANDERSSON K (2018) Erectile Physiological and Pathophysiological Pathways Involved in Erectile DysfunctionJournal of Urology, VOL. 170, NO. 2S, (S6-S14), Online publication date: 1-Aug-2003.SPEKTOR M, RODRIGUEZ R, ROSENBAUM R, WANG H, MELMAN A and CHRIST G (2018) Potassium Channels and Human Corporeal Smooth Muscle Cell Tone: Further Evidence of the Physiological Relevance of the Maxi-K Channel Subtype to the Regulation of Human Corporeal Smooth Muscle Tone In VitroJournal of Urology, VOL. 167, NO. 6, (2628-2635), Online publication date: 1-Jun-2002.PARK J, KIM S, KIM S, KIM Y and CHO K (2018) RENIN ANGIOTENSIN SYSTEM OF RABBIT CLITORAL CAVERNOSUM: INTERACTION WITH NITRIC OXIDEJournal of Urology, VOL. 164, NO. 2, (556-561), Online publication date: 1-Aug-2000.MELMAN A and GINGELL J (2018) THE EPIDEMIOLOGY AND PATHOPHYSIOLOGY OF ERECTILE DYSFUNCTIONJournal of Urology, VOL. 161, NO. 1, (5-11), Online publication date: 1-Jan-1999.GIRALDI A, SERELS S, AUTIERI M, MELMAN A and CHRIST G (2018) ENDOTHELIN-1 AS A PUTATIVE MODULATOR OF GENE EXPRESSION AND CELLULAR PHYSIOLOGY IN CULTURED HUMAN CORPORAL SMOOTH MUSCLE CELLSJournal of Urology, VOL. 160, NO. 5, (1856-1862), Online publication date: 1-Nov-1998.Sullivan M, Dashwood M, Thompson C, Muddle J, Mikhailidis D and Morgan R (2018) ALTERATIONS IN ENDOTHELIN B RECEPTOR SITES IN CAVERNOSAL TISSUE OF DIABETIC RABBITS: POTENTIAL RELEVANCE TO THE PATHOGENESIS OF ERECTILE DYSFUNCTIONJournal of Urology, VOL. 158, NO. 5, (1966-1972), Online publication date: 1-Nov-1997.Montorsi F, Luigi G, Strambi L, Da Pozzo L, Nava L, Barbieri L, Rigatti P, Pizzini G and Miani A (2018) RECOVERY OF SPONTANEOUS ERECTILE FUNCTION AFTER NERVE-SPARING RADICAL RETROPUBIC PROSTATECTOMY WITH AND WITHOUT EARLY INTRACAVERNOUS INJECTIONS OF ALPROSTADIL: RESULTS OF A PROSPECTIVE, RANDOMIZED TRIALJournal of Urology, VOL. 158, NO. 4, (1408-1410), Online publication date: 1-Oct-1997. Volume 153Issue 6June 1995Page: 1998-2003 Advertisement Copyright & Permissions© 1995 by American Urological Association, Inc.MetricsAuthor Information George J. Christ Requests for reprints: Urology Research Laboratory, Room 216S, Forchheimer Building, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, New York 10461. More articles by this author Seth E. Lerner More articles by this author Daniel C. Kim More articles by this author Arnold Melman More articles by this author Expand All Advertisement PDF downloadLoading ...