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Artigo Revisado por pares
BIBTEX RIS

Phosphoenolpyruvate carboxykinase from Tetrahymena pyriformis

1966; Elsevier BV; Volume: 122; Issue: 2 Linguagem: Inglês

10.1016/0926-6593(66)90083-x

ISSN

1878-1454

Autores

E. Shrago, A. L. Shug,

Tópico(s)

Electrochemical sensors and biosensors

Resumo

1.1. Tetrahymena pyriformis has two isoenzymes of malate dehydrogenase, I and II. After purifying (isoenzyme I, 85-fold), the mol. wt of each enzyme was determined to be around 122,000 with four subunits of 28,000.2.2. The Michaelis constants are: 0.25 mM for oxaloacetate (isoenzyme I), 0.8 mM for oxaloacetate (isoenzyme II), 4mM for malate (isoenzyme I and Il), 0.13 mM for NAD (isoenzyme I and II) and 0.24 mM for NADH (isoenzyme I and II).3.3. It was concluded that Tetrahymena malate dehydrogenase was closer to the enzyme from Gram positive bacteria than to that from eukaryotes.

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