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Immunosuppressive and anti-inflammatory effects of methanolic extract and the polyacetylene isolated from Bidens pilosa L.

1999; Elsevier BV; Volume: 43; Issue: 1 Linguagem: Inglês

10.1016/s0162-3109(99)00039-9

1879-047X

Rachel L.C Pereira, Tereza Ibrahim, Leonardo Lucchetti, Antônio Jorge Ribeiro da Silva, Vera Lúcia Gonçalves de Moraes,

Sesquiterpenes and Asteraceae Studies

The immunomodulatory effect of the methanolic extract obtained from dried leaves of Bidens pilosa L. (Asteraceae) and the polyacetylene 2-O-β-d-glucosyltrideca-11E-en-3,5,7,9-tetrayn-1,2-diol (PA-1) isolated from it was investigated. The extract inhibited the proliferative response in two in vitro models: human lymphocytes stimulated by 5 μg ml−1 phytohemagglutinin (PHA) or to 100 nM 12-O-tetradecanoyl phorbol-13-acetate (TPA) plus 0.15 μM ionomycin and murine lymphocytes stimulated by 5 μg ml−1 concanavalin A (Con A) or in the mixed leukocyte reaction (IC50=12.5 to 25 μg ml−1). PA-1 was 10-fold more potent than the original extract in blocking both human and murine lymphocyte proliferation (IC50=1.25 to 2.5 μg ml−1). In mice, the intraperitoneal (i.p.) administration of methanolic extract of B. pilosa significantly reduced the size of the popliteal lymph node (PLN) after the inflammation induced by zymosan. One week after the injection of zymosan (150 μg) in the foot pad, PLN weighed 4.6±0.6 mg in comparison with 0.5±0.07 mg of the contralateral non-inflamed foot pad. The i.p. treatment with 10 mg extract from day 2 to day 6 after zymosan injection reduced the PLN weight to 1.8±0.3 mg. The data suggest an immunosuppressive activity of components of B. pilosa that may explain its popularly perceived anti-inflammatory effect.