Artigo Revisado por pares

Biosensors: Fundamentals, applications and trends

1991; Elsevier BV; Volume: 4; Issue: 1-2 Linguagem: Inglês

10.1016/0925-4005(91)80198-s

ISSN

1873-3077

Autores

F Scheller, R. Hintsche, D. Pfeiffer, F. Schubert, K. Riedel, R. Kindervater,

Tópico(s)

Analytical Chemistry and Sensors

Resumo

Enzyme electrodes and optical immunosensors are at the leading edge of the biosensor field. The sensitivity of multi-enzyme electrodes is shifted to the subnanomolar concentration range. With monoenzyme sensors, several thousand samples per hour are measurable. Direct as well as enzyme-labelled immunosensors nowadays reach the sensitivity of immunoassays. The effect of non-specific binding is the biggest challenge for further improvement. Using multi-enzyme systems or intact cells, biologically related parameters, e.g., taste, odour, fatigue substances, mutagenicity, allergenicity and biological oxygen demand are quantifiable. Further progress is expected by applying tailor-made enzymes, antibodies and neuronal networks.

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