Rhamnose-binding Lectins from Steelhead Trout ( Oncorhynchus mykiss ) Eggs Recognize Bacterial Lipopolysaccharides and Lipoteichoic Acid
2002; Oxford University Press; Volume: 66; Issue: 3 Linguagem: Inglês
10.1271/bbb.66.604
ISSN1347-6947
AutoresHiroaki Tateno, Tomohisa Ogawa, Koji Muramoto, Hisao Kamiya, Mineo Saneyoshi,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoThe interaction between bacteria and three L-rham-nose-binding lectins, named STL1, STL2, and STL3, from steelhead trout (Oncorhynchus mykiss) eggs was investigated. Although STLs bound to most Gram-negative and Gram-positive bacteria, they agglutinated only Escherichia coli K-12 and Bacillus subtilis among the bacteria tested. The binding was inhibited by L-rhamnose. STLs bound to distinct serotypes of lipopolysaccharides (LPSs), and showed much higher binding activities to smooth-type LPSs of Escherichia coli K-12 and Shigella flexneri 1A than to their corresponding rough-type LPSs. STLs also bound to lipoteichoic acid (LTA) of Bacillus subtilis. These results indicate that STLs bound to bacteria by recognizing LPSs or LTA on the cell surfaces.
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