Estimation by radiation inactivation of the minimum functional size of acrosome-reaction-including substance (ARIS) in the starfish, Asterias amurensis
1995; Cambridge University Press; Volume: 3; Issue: 4 Linguagem: Inglês
10.1017/s0967199400002781
ISSN1469-8730
AutoresAkira Ushiyama, Kazuyoshi Chiba, Akihiro Shima, Motonori Hoshi,
Tópico(s)Isotope Analysis in Ecology
ResumoSummary In the starfish Asterias amurensis , the jelly coat of the eggs contains a glycoprotein essential for the induction of the acrosome reaction in homologous spermatozoa that is termed the acrosome-reaction-inducing substance (ARIS).ARIS is a highly sulphated and fucose-rich glycoprotein of extremely high molecular mass(>10 4 kDa). ARIS was irradiated with high-energy eletrons in order to estimate the minimum size required for its biological activity. The minimum functional unit or target size of ARIS was estimated to be c . 14 kDa by target size analysis. ARIS was significantly disintegrated by the irradiation, yet the total sugar content was not apparently reduced. The binding of 125 I-labelled ARIS to spermatozoa competed with that of irradiated ARIS, although the affinity of ARIS was much reduced after irradiation.
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