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BIBTEX RIS

Understanding the cellulolytic system of Trichoderma harzianum P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase

2012; Elsevier BV; Volume: 131; Linguagem: Inglês

10.1016/j.biortech.2012.12.105

1873-2976

Priscila da Silva Delabona, Júnio Cota, Zaira B. Hoffmam, Maria Paula Paixão, Cristiane S. Farinas, João Paulo L. Franco Cairo, Deise Juliana da Silva Lima, Fábio M. Squina, Roberto Ruller, José Geraldo da Cruz Pradella,

Microbial Metabolites in Food Biotechnology

Supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. This study investigated which enzymes were complementary to the enzyme set of Trichoderma harzianum in the degradation of sugarcane bagasse. Specific activities of T. harzianum extract on different substrates were compared with the extracts of Penicillium echinulatum and Trichoderma reesei, and two commercial cellulase preparations. Complementary analysis of the secretome of T. harzianum was also used to identify which enzymes were produced during growth on pretreated sugarcane bagasse. These analyses enabled the selection of the enzymes pectinase and α-l-arabinofuranosidase (AF) to be further investigated as supplements to the T. harzianum extract. The effect of enzyme supplementation on the efficiency of sugarcane bagasse saccharification was evaluated using response surface methodology. The supplementation of T. harzianum enzymatic extract with pectinase and AF increased the efficiency of hydrolysis by up to 116%.