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A 1.5-Megabase Physical Map Encompassing the Multiple Endocrine Neoplasia Type-1 (MEN1) Locus on Chromosome 11q13

1996; Elsevier BV; Volume: 38; Issue: 2 Linguagem: Inglês

10.1006/geno.1996.0612

1089-8646

Thomas F. Wood, Eri S. Srivatsan, Rita Chakrabarti, C. Gary, Naikang Kuan, Ghassan Samara, Michael J. Higgins, T.B. Shows, Carey L. Johnson, Yu‐Jui Yvonne Wan, Edward Passaro, Mark Sawicki,

Glycosylation and Glycoproteins Research

Linkage analysis and loss of heterozygosity studies have shown that the gene responsible for the multiple endocrine neoplasia type-1 (MEN1) syndrome localizes to a small interval between D11S427 and D11S460 on chromosome 11q13. As an initial step to clone this tumor suppressor gene, our group is the first to map the MEN1 region physically using yeast artificial chromosome, bacterial artificial chromosome (BAC), and cosmid contigs. The 1.5-Mb high-resolution, contiguous map extends from PYGM to 300 kb telomeric of D11S460. Of this, the 1.2-Mb interval between PYGM and D11S460 is isolated in cosmids and BACs and will be useful for the development of genomic sequences and transcription maps of this important region. Nine new sequence-tagged sites (STS) are also characterized from this region. The physical map and the STSs will be valuable tools for the cloning of the MEN1 gene.