Combined enzymatic synthesis of nucleotide (deoxy) sugars from sucrose and nucleoside monophosphates
1996; Elsevier BV; Volume: 52; Issue: 7 Linguagem: Inglês
10.1016/0040-4020(95)01081-5
ISSN1464-5416
AutoresAstrid Zervosen, Andréas Stein, Holger Adrian, Lothar Elling,
Tópico(s)Amino Acid Enzymes and Metabolism
ResumoThe synthesis of NDP-glucose 3a-d (NA, C, U, dU) with sucrose synthase B was combined with the enzymatic synthesis of nucleoside diphosphates 2a-d from their corresponding nucleoside monophosphates 1a-d by different kinases A. Further combination with recombinant dTDP-glucose 4,6-dehydratase D enabled us to synthesize dUDP-6-deoxy-α-D-xylo-4-hexulose 5 from 1d on a preparative scale. By using the repetitive batch technique the enzymatic syntheses of nucleotide (deoxy) sugars 3a-d, 5 could be realized on a 0.1 – 0.5 g scale.
Referência(s)