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Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes

2005; Instituto de Tecnologia do Paraná (Tecpar); Volume: 48; Issue: spe2 Linguagem: Inglês

10.1590/s1516-89132005000700035

1678-4324

Andréa C. Alves, Cristiano Machado Gontijo, Mônica Cristina Oliveira, Simone Odília Antunes Fernandes, Flávia Márcia Oliveira, Valbert Nascimento Cardoso, Gilson Andrade Ramaldes,

RNA Interference and Gene Delivery

The oral administration of proteic antigens, like ovalbumin, may result in the induction of oral tolerance or immunization. The aim of this work was to label a protein antigen with 99mTechnetium, encapsulate it in liposomes and investigate its absorption and tissue distribution after oral administration in mice. Ovalbumin was labeled with 99mTechnetium and encapsulated in small unilamellar vesicles. 99mTc-OVA encapsulated or not in liposomes was administrated to mice that were sacrificed after different times. The radioactivity was measured in various organs of the animals. Differences concerning the biodistribution of 99mTc-OVA were noticed. The technique may represent alternatives for the induction of immunization or oral tolerance.