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Structural studies of cell surface and secreted IgG in LPS-stimulated murine B cells

1983; Elsevier BV; Volume: 20; Issue: 4 Linguagem: Inglês

10.1016/0161-5890(83)90018-4

1872-9142

Dorothy Yuan, Ellen S. Vitetta,

Glycosylation and Glycoproteins Research

We have characterized the cell surface IgG of LPS-stimulated B lymphocytes by surface iodination and specific immunoprecipitation. IgG3 was found to be the predominant IgG subclass expressed on the B cell surface after 5 days of stimulation with LPS. In contrast. IgG2b, IgG3, and IgG1 subclasses are all secreted. Cell surface IgG exhibits detergent binding characteristics not shown by secreted IgG and the y chains of the three subclasses of surface IgG examined have the apparent mol. wt of 64 Kd. some 9.000 larger than the H chains of secreted IgG.