Regeneration of Coryphantha elephantidens (Lem.) Lem. (Cactaceae) from root explants
1999; Elsevier BV; Volume: 81; Issue: 3 Linguagem: Inglês
10.1016/s0304-4238(99)00005-9
ISSN1879-1018
Autores Tópico(s)Plant Pathogens and Fungal Diseases
ResumoRegeneration of Coryphantha elephantidens (Lem.) Lem. from callus cultures was obtained from (5–20 mm long) root explants of in vitro rooted shoots cultivated on the Murashige–Skoog medium, supplemented with different concentrations of growth regulators. Callus proliferation and shoot formation was optimal on a medium supplemented with 9 μM 2,4-D and 4.6 μM Kn. Explant size and sucrose concentration in the medium influenced the regeneration potential of the callus cultures significantly. Callus obtained from 10 mm long explant when cultured on a medium supplemented with 9 μM 2,4-D, 4.6 μM Kn and 7% sucrose induced maximum shoot differentiation. Regenerants were rooted on growth-regulator-free Murashige–Skoog medium and then acclimatized in a greenhouse. Regenerated plants showed 100% survival and performed better than seedlings.
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