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Diterpenoids and flavonoids from the fruits of Vitex agnus‐castus and antioxidant activity of the fruit extracts and their constituents

2007; Wiley; Volume: 21; Issue: 4 Linguagem: Inglês

10.1002/ptr.2021

1099-1573

Zsuzsanna Hajdú, Judit Hohmann, Péter Forgó, Tamás A. Martinek, Máté Dervarics, István Zupkó, György Falkay, Dániel Cossuta, Imre Máthé,

Plant-based Medicinal Research

Abstract From the n ‐hexane fraction of the fruits of Vitex agnus‐castus , two labdane‐type diterpenes, vitetrifolin B and C, were isolated by means of multiple chromatographic separations, together with the previously identified rotundifuran, vitexilactone and the sesquiterpene spathulenol. From the EtOAc fraction, eupatorin was identified for the first time, besides the known casticin, penduletin, vitexin and orientin. The n ‐hexane, EtOAc and MeOH–H 2 O fractions of the MeOH extract of Agni‐casti fructus were subjected to in vitro antioxidant assays. The EtOAc extract displayed a significant concentration‐dependent effect when tested by 1,1‐diphenyl‐2‐picrylhydrasyl (DPPH) free radical assay (IC 50 = 68 µg/mL) and against the autooxidation of a standard rat brain homogenate (IC 50 = 14 µg/mL). The MeOH–H 2 O fraction was less active with 3643 µg/mL (DPPH test) and IC 50 = 125 µg/mL (rat brain homogenate), while the n ‐hexane phase proved to be inactive. The main flavonoid constituents of the EtOAc extract, casticin, vitexin and orientin were assayed for antioxidant activity and found that only casticin possesses a marked lipid peroxidation inhibitory effect (IC 50 = 0.049 m m ) compared with that of the positive control ascorbic acid (IC 50 = 0.703 m m ). Copyright © 2007 John Wiley & Sons, Ltd.